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PITTCON 08 Oral Session
Quantitation of carbohydrates and uronic acids in Eucalyptus sp wood by ion chromatography with pulsed amperometric detection using a gold electrode
Session Number 2740-4
Abstract The determination of carbohydrates and uronic acids in Eucalyptus sp woods is of crucial importance for improving the industrial process of paper production. Moreover, important information concerning the biochemical characteristics of the used wood can be obtained. The purpose of this study was to quantify the carbohydrate (arabinose, galactose, glucose, xylose and manose) and uronic acid (glucuronic and galacturonic acids) content in Eucalyptus sp wood powder. Prior to injection, the target compounds were extracted from the Eucalyptus sp wood powder by acid digestion. Separation of the analytes was performed on a high-capacity ion-exchange column using a sodium hydroxide/sodium acetate eluent. Detection of the carbohydrates and uronic acids was performed with a pulsed amperometric detector using a gold electrode. This communication describes carbohydrate and uronic acid analysis using rapid, innovative and sensitive ion chromatography. Detection limits are in the range of 1 – 2.5 ppm. 70% (w/w) of the dry wood powder consist of glucose and xylose and only 1 – 2% of uronic acids. Regarding the uronic acids, the concentration of galacturonic acid exceeds that of glucuronic acid.
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